Usage: Paste a DNA sequence, ID or AC (ex. U73445) and the two primers (ex. forward AAA[AT]AA and reverse AAAGGG), select the format, and the options and click the "Run iPCR" button.
The primer syntax allows the square parenthesis ([]) to specify an alternative and the dot (.) to replace the N for any nucleotide. The degenerated code is not allowed.

This tool is not a primer "finder", if you are looking for such a tool, go to the Primer3 web site.

Make sure that the format button shows the correct format.

Select format:

Query title (option)

Paste your sequence here:
(or ID or accession number)

(5'->3')

(5'->3')

Options:

(bp)

(bp)